一种5 重real-time PCR筛查转基因水稻方法的建立

以转基因水稻中最常用的CaMV35S启动子、NOS终止子、Cry1Ab/Ac基因、HPT基因及SPS水稻内标基因为研究对象，利用5 种不同的荧光信号（FAM、HEX、Taxas Red、Cy5、Cy5.5）进行多重实时聚合酶链式反应（real-time polymerase chain reaction，real-time PCR）检测方法的研究。通过引物组合筛选、反应体系优化、特异性测试、灵敏度测试、适用性测试等一系列实验，建立了5 重real-time PCR方法，灵敏度可达0.032%。此方法具有灵敏度高、结果准确、通量大等优点，可实现水稻中转基因成分的快速、高效检测。     

Quasi-static compressive behaviour of aluminium cenosphere syntactic foams

In this paper, cenosphere particles embedded in AA2014 aluminium matrix are used to fabricate syntactic foam by stir casting method. The particle size is about 100?µm and foam density is about 1990?kg?m^?3. Compression tests at strain rate 0.001/s are performed on foam samples to characterise their mechanical properties which are then used in numerical analysis on commercial finite element analysis software ABAQUS/CAE with isotropic elastic-plastic material model. Experimental and numerical results show good conformity in deformation behaviour with elastic and plateau zones showing average deviations less than 5% and 20%, respectively. Foams showed high yield stress and energy absorption capabilities that can be useful in making blast and impact resistant structures.     

On the thermally-induced seizure in bearings: A review

This paper presents a state-of-the-art survey of papers reported on the nature of a troublesome failure mode in bearings known as seizure. This mode of failure is thermally-induced and it occurs in both journal and rolling element bearings. To gain insight, particular attention is given to reported experimental observation, various mechanisms involved, and available prediction methodologies.     

A high performance crashworthiness simulation system based on GPU

Crashworthiness simulation system is one of the key computer-aided engineering (CAE) tools for the automobile industry and implies two potential conflicting requirements: accuracy and efficiency. A parallel crashworthiness simulation system based on graphics processing unit (GPU) architecture and the explicit finite element (FE) method is developed in this work. Implementation details with compute unified device architecture (CUDA) are considered. The entire parallel simulation system involves a parallel hierarchy-territory contact-searching algorithm (HITA) and a parallel penalty contact force calculation algorithm. Three basic GPU-based parallel strategies are suggested to meet the natural parallelism of the explicit FE algorithm. Two free GPU-based numerical calculation libraries, cuBLAS and Thrust, are introduced to decrease the difficulty of programming. Furthermore, a mixed array and a thread map to element strategy are proposed to improve the performance of the test pairs searching. The outer loop of the nested loop through the mixed array is unrolled to realize parallel searching. An efficient storage strategy based on data sorting is presented to realize data transfer between different hierarchies with coalesced access during the contact pairs searching. A thread map to element pattern is implemented to calculate the penetrations and the penetration forces; a double float atomic operation is used to scatter contact forces. The simulation results of the three different models based on the Intel Core i7-930 and the NVIDIA GeForce GTX 580 demonstrate the precision and efficiency of this developed parallel crashworthiness simulation system.     

面向流屑角突变建模的切削状态参数经验公式的获得及应用

从提高流屑角突变模型的预测精度出发,建立了切削状态参数与3个切削控制参数之间关系的新经验公式。提出了一种通过迭代法准确设定有限元仿真软件刀-屑摩擦因数的方法,并通过直角切削Al6061-T6工件的有限元仿真试验,获得了一组不同切削控制参数组合条件下的切削状态参数数据。根据该数据拟合出剪切角φ、刀-屑摩擦角β、剪切应力τs关于刀具前角γ0、进给量f和切削速度v的经验公式,并通过一组直角切削试验,验证了所得经验公式的有效性。将新建经验公式应用于流屑角突变建模过程后,所得模型关于突变临界切削宽度的平均预测误差减小了27.2%。     

Homogenization with uncertainty in Poisson ratio for polymers with rubber particles

The main aim of this work is dual computer analysis of probabilistic coefficients for the homogenized tensor of the polymer filled with the rubber particles having randomized Poisson ratios of both constituents. The major issue is to verify an influence of a randomness in rubber Poisson ratio close to the compressibility limit on the uncertainty of the effective tensor probabilistic characteristics. Probabilistic analysis presented here is carried out using mainly the stochastic perturbation technique provided by the common application of the traditional FEM commercial code ABAQUS and the symbolic computations package MAPLE. This FEM-based technique employs polynomial response function of the optimum order recovered from the weighted least squares method and following a set of deterministic solutions obtained for various values of the randomized input parameter. Optimization procedure is released entirely into a symbolic environment, where maximization of the correlation factor together with minimization of the fitting variance and approximation error are applied. Homogenization technique consists in equating of deformation energies for the real composite and the artificial one characterized by the effective elasticity tensor with uncertainty.     

钛基Ce掺杂SnO_2-RuO_2电极的制备及其对焦化废水的电解处理

采用溶胶-凝胶法制备了经稀土元素Ce掺杂改性的Ti/RuO_2-SnO_2-Ce电极,利用SEM、XRD、EDS等分析方法对电极表面的形貌、物相和涂层成分进行了表征,利用CV曲线、LSV曲线等检测手段对电极的电化学性能进行了测试。以Ti/RuO_2-SnO_2-Ce电极为阳极,钛板为阴极,构建电化学氧化反应装置,并采用其对焦化废水进行电解处理。结果表明,Ti/RuO_2-SnO_2电极经稀土Ce改性后极板表面活性位点数量明显增大,金属涂层的结晶化程度明显增强,电极的催化氧化活性显著提高。室温条件下,保持pH值为7.83,在电流密度为30mA/cm~2,电解时间为30min时,焦化废水经电解处理后COD去除率可达91.63%,TOC去除率可达66.22%,UV254值下降到0.921。     

Design and Fabrication of Concentration‐Gradient Generators with Two and Three Inlets in Microfluidic Chips

A simple and low‐cost method for designing and fabricating concentration‐gradient generators with two and three inlets is proposed which can generate different concentration gradients at varying flow velocities. The microchannel structure was designed in S‐shape and left‐right symmetry. The concentration‐gradient generator was simulated based on the finite element method. The microchannels were processed on a computer numerical control (CNC) engraving and milling machine on poly(methylmethacrylate) substrate, and then two concentration‐gradient generators were fabricated by hot bonding technology. The results of experiment and simulation were compared to prove the feasibility of the method. Flow velocity was an important factor for generating different concentration gradients. The concentration‐gradient profiles of the generators with two and three inlets present approximately linear and quadratic curves.